Figure 3

Rapid deterioration of survival proteins and increased apoptosis in STZ-induced female diabetic heart. A-B and D-E representative blots and bar graphs showing the levels of Pim-1 (A), total and cleaved Caspase-3 (B), pAkt/Akt (D) and Bcl-2 (E) in hearts of diabetic and non-diabetic hearts of both the genders at different time points after induction of diabetes (n = 6 at each time point). C representative immunofluorescence image showing TUNEL positive cardiomyocytes at 12 weeks after STZ-induced diabetes. Quantitative bar graphs showing the percentage of TUNEL positive cardiomyocytes at different time points after induction of diabetes (n = 6 at each time point). F-G Bar graphs showing the expression level of miR-1 (F) and miR-208 (G) in study groups (n = 5 at each time point). Values are means ± SD. Results of pair-wise comparison are illustrated. *P < 0.05, **P < 0.01 and ***P < 0.001 vs. non-diabetic of corresponding gender at corresponding time point. ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 vs. male diabetic at corresponding time point.