Figure 5

LOX-1 and TNF-α expression in bovine aortic endothelial cells (BAECs) treated with LDL and/or estrogens. A, Representative Western blot showing LOX-1 and TNF-α protein expression in BAECs treated with LDL from male db/db mice (mdbLDL) in the presence or absence of pretreatment with 100 nM genistein (Gen) or 10 nM 17β-estradiol (E2). B, Quantification of protein expression shown in (A), expressed as a percent ratio to the expression of β-actin (internal loading control) (n = 4). *P < 0.05 vs control-treated BAECs, ^#P < 0.05 vs. mdbLDL-treated BAECs.