Effect of LDL electronegativity and estrogens on bovine aortic endothelial cell (BAEC) senescence. A, Representative images of SA-β-gal staining of senescent BAECs (blue) 5 days after incubation with 30 μg/mL LDL isolated from male or female db/db or wild-type mice in the presence or absence of pretreatment with 17β-estradiol (10 nM) or genistein (100 nM). B, The percentage of senescent cells and (C) telomerase activity in BAECs treated with LDL from male or female db/db or wild-type mice in the presence or absence of 17β-estradiol or genistein. n = 4 for each group. *P < 0.01 vs. gender-matched wild-type LDL, #P < 0.05 and ##P < 0.01 vs. gender-matched db/db LDL.