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Figure 4 | Cardiovascular Diabetology

Figure 4

From: Advanced glycation end products potentiate citrated plasma-evoked oxidative and inflammatory reactions in endothelial cells by up-regulating protease-activated receptor-1 expression

Figure 4

Effects of FR171113 or rivaroxaban on superoxide generation (A), RAGE (B), MCP-1 (C), ICAM-1 (D) gene expression in, and THP-1 cell adhesion to (E), HUVECs. HUVECs were pre-incubated with or without 30 nM ribaroxaban for 30 min and treated in the presence or absence of 3% citrated human plasma or 1 mM FR171113 for 4 hr. (A) Then the cells were incubated with DHE. ROS generation was evaluated by fluorescent intensity. N = 5 per group. (B-D) Total RNAs were transcribed and amplified by real-time PCR. Data were normalized by the intensity of β-actin (B) or 18S mRNA-derived signals ((C) and (D)) and then related to the value obtained with non-glycated BSA alone. (E) THP-1 cell adhesion to HUVECs was evaluated. (B) N = 7 per group. (C) and (D) N = 3 per group. (E) N = 6 per group. * and **, p < 0.05 and p < 0.01 compared to the value with BSA + 3% human plasma alone, respectively.

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Cardiovascular Diabetology

ISSN: 1475-2840