Figure 12

Measurement of MMPs activity in mesenteric arteries: MMPs activity was measured using in situ zymography performed in arterial thin sections submitted to angiotensin II (angII) or not (control or Cont). Gelatinase activity was visualized as an enhanced fluorescence of a fluorogenic gelatin substrate in mesenteric arteries using confocal microscopy. Arteries were isolated from LZ rats or from ZDF rats treated or not with ALT-711, TEMPOL or TEMPOL + ALT-711. Bargraph: Quantification of gelatinolytic activity was performed using image density analysis (3–4 arterial sections per artery and 6 rats per group). Mean ± SEM is presented. *P < 0.05, Angiotensin II versus control.