Figure 5
From: Impaired dopamine D1 receptor-mediated vasorelaxation of mesenteric arteries in obese Zucker rats
Effect of insulin and glucose on D 1 receptor expression and phosphorylation in A10 cells. A10 cells were treated with varying concentrations of insulin (A and B) or glucose (C and D) for 24 hrs. D1 receptor expression was determined by immunoblotting. A10 cells were treated with insulin (10-7M) and glucose (35 mM) for 24 hrs. D1 receptor expression and phosphorylation were determined by co-immunoprecipitation or immunoblotting. D1 receptor serine phosphorylation was normalized by D1 receptor protein (*P < 0.05, vs. control, n = 5).