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Figure 6 | Cardiovascular Diabetology

Figure 6

From: Vaspin inhibits cytokine-induced nuclear factor-kappa B activation and adhesion molecule expression via AMP-activated protein kinase activation in vascular endothelial cells

Figure 6

AMPK activation mediates the inhibitory effects of vaspin on TNFα-induced adhesion of monocytes to HAECs. The effect of vaspin on TNFα-induced adhesion of monocytes to HAECs was measured by staining with VibrantDiO® Cell-Labeling Solution at 4 hr after treatment with 100 ng/mL vaspin in the presence of absence of 10 ng/mL TNFα. HAECs were transfected with control siRNA (A) or AMPKα1 siRNA (B) 24 hr before. Data shown are representative microphotographs (top panels) and mean values ± SEM of three independent experiments (bottom panels). Green fluorescence was visualized using confocal microscopy. Magnification, × 40. *p < 0.05 vs. untreated cells, †p < 0.05 vs. cells treated with vaspin alone, and §p < 0.05 vs. cells treated with TNFα alone.

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Cardiovascular Diabetology

ISSN: 1475-2840