Figure 4

Vaspin inhibits TNFα-induced adhesion of monocytes to HAECs. The effect of vaspin on TNFα-induced THP-1 cell adhesion to HAECS measured using a static adhesion assay (A) and staining with VibrantDiO® Cell-Labeling Solution (B and C). The level of THP-1 cell adhesion to HAECs was measured at 4 hr after treatment with 25–100 ng/mL vaspin in the presence or absence of 10 ng/mL TNFα. BAY11-7082 (10 μM, S2913, Selleckchem, USA), an inhibitor of NF-κB, was used as a positive control. In A and C, data shown are mean values ± SEM of three independent experiments. Representative microphotographs are shown in B, and these data are quantified in C. Green fluorescence was visualized using confocal microscopy. Magnification, × 40. ^*p < 0.05 vs. untreated cells, ^†p < 0.05 vs. cells treated with TNFα alone, and ^§p < 0.05 vs. cells treated with 25 ng/mL vaspin.