Figure 4

Inflammatory cell infiltration in mesenteric perivascular adipose tissue (PVAT). A, Representative images of CD45 immunohistochemical staining of a small mesenteric artery and surrounding PVAT. The number of CD45-positive cells was not different between I-MetS mice and controls, but it was increased in MetS mice (data are presented as mean ± SEM, n = 6 mice/group, *P < 0.05, scale bars = 50 μm). B, Representative images of two-photon microscopy of a small mesenteric artery on the left, with the surrounding PVAT stained for CD45/CD115 and CD45/Gr-1. I-MetS mice did not present with increased number of positive cells. In MetS mice there was a trend toward more positive cells (P = 0.06, scale bars = 100 μm). The column graph represents the number of positive cells per arterial segment examined (data are presented as mean ± SEM, 3 segments/mouse, n = 4 mice/group). C, In contrast with MetS mice, I-MetS mice did not present increased expression of inflammatory genes in mesenteric PVAT. Results represent three independent experiments, n = 3–4 mice/group, data are presented as mean ± SEM, *P < 0.05, **P < 0.01.