Figure 4From: Azilsartan, an angiotensin II type 1 receptor blocker, restores endothelial function by reducing vascular inflammation and by increasing the phosphorylation ratio Ser1177/Thr497 of endothelial nitric oxide synthase in diabetic miceGene expression in aorta (upper panel), perivascular fat (middle panel), and soleus muscle (lower panel). Samples isolated from male KKAy mice fed standard moderate-fat (MF) diet (black bars) and MF diet mixed with 0.005% candesartan cilexetil (gray bars) or 0.005% azilsartan (shaded bars) for 3 weeks since 8–10 weeks of age, were analyzed by semiquantitative RT-PCR. Gapdh was used as an internal control. Values shown represent mean ± SEM (n = 4–6).†p < 0.05 compared to C57BL/6 J and +p < 0.1 and *p < 0.05 compared to KKAy-vehicle according to either the Kruskal–Wallis test or unpaired t test. TNFα: tumor necrosis factor α; MCP1: monocyte chemotactic protein 1; PPARγ: peroxisome proliferator–activated receptor γ2; IRS-1: insulin receptor substrate 1; and Adipo: adiponectin.Back to article page