Figure 8

Levels of phosphorylated AMPK and ACC in the hearts of 60-week old liver-specific gck knockout mice. Representative Western blot images (A) and quantification of the levels of p-AMPKα/AMPKα (B), p-AMPKβ/AMPKβ (C), and p-ACC/ACC (D) in heart homogenates of wild-type (gck^w/w) and gck knockout (gck^w/–) mice as well as knockout mice treated with insulin or rosiglitazone for 4 weeks are shown. Phosphorylated AMPK and ACC levels were measured by Western blots. n = 3 for all samples. Asterisk (*) refers to statistical significance (P < 0.05) in comparisons with gck^w/– mice, while # refers to comparisons with gck^w/w mice.