Figure 5

Cardiac fibrotic levels in 60-week old liver-specific gck knockout mice. Cardiac fibrosis levels were investigated in wild-type (gck^w/w) and gck knockout (gck^w/–) mice as well as knockout mice treated with insulin or rosiglitazone for 4 weeks. Images of PAS and Masson staining are shown in (A) with positive Masson IOD/area (B), and positive PAS IOD/area (C) shown. The relative amount of mRNA for fibronectin 1 in the myocardial, which was determined by real-time PCR and corrected with beta actin as an internal standard, is shown in (D). n = 3 for all samples. Asterisk (*) refers to statistical significance (P < 0.05 for * and P < 0.001For ***) in comparisons with gck^w/– mice, while # refers to statistical significance (P < 0.005 for ## and P < 0.001 for ###) in comparisons with gck^w/w mice.