Figure 4
From: An accelerated mouse model for atherosclerosis and adipose tissue inflammation
A sucrose-rich high-fat diet for 16 weeks increases adipose tissue inflammation in obese LDLR-/-mice. LDLR-/- mice were fed HFSC, HFC or LF for 16 or 20 weeks (n = 6 animals per group). ApoE-/- mice were fed HFSC for 16 weeks (n = 8 animals per group). Gonadal adipose tissue expression of the genes for macrophage marker F4/80 (encoded by Emr1) (A), TNFα (Tnf) (B), IL-6 (Il6) (C), MCP-1 (Ccl2) (D), osteopontin (OPN; Spp1) (E) and adiponectin (AdipoQ) (F) was analyzed by real-time RT-PCR. Plasma osteopontin was determined by a commercially available ELISA (G). For statistical analysis LDLR-/- mice fed HFSC or LF were compared with HFC-fed LDLR-/- mice. In addititon, LDLR-/- and ApoE-/- mice both fed HFSC were compared. All data represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.