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Figure 2 | Cardiovascular Diabetology

Figure 2

From: Reduced levels of circulating endothelial progenitor cells in acute myocardial infarction patients with diabetes or pre-diabetes: accompanying the glycemic continuum

Figure 2

Representative flow cytometry quantification of CD45low/CD34+/KDR+ EPCs in a a nondiabetic patient with STEMI. (A) The cluster of CD34+ cells (red points) was analyzed using FITC-labeled antibodies against CD34 vs. SSC. B) CD45dim subset analysis was conducted using APC-H7-labeled antibodies against CD45 vs SSC. At this step, events forming a cluster of characteristic low SSC and low CD45 fluorescence (SSClowCD45dim cells) expressed CD34+CD45dim cells (light blue events). C) The events fulfilling previous criteria were then displayed on a forward light scatter (FSC) vs. SSC dot plot to confirm that the selected cells fall into the lymphocyte region. D) Finally, CD45dimCD34+KDR+ endothelial progenitor cells (pink cells) are deducted in the dot plot of PE-labeled antibodies against KDR vs CD34.

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Cardiovascular Diabetology

ISSN: 1475-2840