Figure 8

Immunofluorescent micrographs of ATP7B localization in sections of the LV free-wall from control, diabetic and TETA-treated diabetic rats. A: Representative confocal images (100 ×-objective) of LV-wall longitudinal sections labeled with anti-ATP7B antibody (red). Sections were co-stained for sarcolemma (green) and nuclei (blue) with WGA-Oregon Green 488 and DAPI, respectively. Arrows indicate the co-staining of nuclei and ATP7B: scale bar = 20 μm. The more highly magnified images show co-localization of ATP7B and T-tubule membranes: scale bar = 4 μm. B: Representative confocal images (60 ×-objective) of longitudinal LV-wall sections labeled for ATP7B (red) and N-cadherin (green). Arrows indicate co-staining at the intercalated disk: scale bar = 30 μm. C: Representative confocal images (40 ×-objective) of transverse LV-wall sections labeled with anti-ATP7B antibody (red), WGA (green), and DAPI (blue). Arrows indicate nuclear/peri-nuclear and sarcoplasmic vesicles: scale bar = 50 μm: n = 40 sectional images/group.