Figure 3From: Diabetic cardiomyopathy is associated with defective myocellular copper regulation and both defects are rectified by divalent copper chelationImmunofluorescent micrographs of CTR2 localization in sections of the LV free-wall from control, diabetic and TETA-treated diabetic rats. A: Representative LV wall cross-sections (100 ×-objective) labeled with anti-CTR2 antibody (RRX red). Arrows indicate ‘vesicle-like’ structures adjacent to the sarcolemmal membrane. B: Representative longitudinal sections of LV-wall (100 ×-objective) labeled with anti-CTR2 antibody (red). Arrows indicate intercalated disks. All sections in A and B were co-stained for sarcolemmal membranes (green) and nuclei (blue) with WGA-Oregon Green 488 and DAPI, respectively: n = 40 sectional images/group: scale bar = 20 μm. C: Representative double-immunofluorescent labeling of CTR2 and N-cadherin in longitudinal LV sections (60×-objective). Arrows indicate co-localization at the intercalated disk: n = 10 sectional images/group: scale bar = 30 μm.Back to article page