Figure 5

Effect of CAPA on concentration-dependent contractile response to phenylephrine in endothelium-denuded aortic strips. The effect of CAPA on the contractile response to phenylephrine was measured in endothelium-denuded thoracic aorta. The aortic strips were pre-treated with 1, 10 and 100 μM CAPA, and phenylephrine was added in a cumulative dose in the organ bath (A). Normalized contraction to the maximum response of phenylephrine is shown (B). The EC₅₀ values for phenylephrine and the Hill coefficient are shown in (C) and (D). Data (mean ± SEM) were obtained from 6–8 animals. *P < 0.05 compared with the vehicle, DMSO pre-treatment group.