Figure 5
From: Role of ROCK upregulation in endothelial and smooth muscle vascular functions in diabetic rat aorta
Phospho/total MYPT1 and Phospho/total MLC expressions in rat thoracic aorta. (A) Representative Western Blots for phospho-MYPT1, MYPT1 (140 kDa) and β-actin expressions from control (CON) and diabetic (DM) rat aorta homogenates. (B) MYPT1 and (C) Phospho-MYPT1 normalized to β-actin. (D) Phospho-MYPT1 was normalized to corresponding MYPT1 protein band, respectively. (E) Representative Western Blots for phospho-MLC, MLC (20 kDa) and β-actin (43 kDa) expressions from CON and DM rat aorta homogenates. (F) MLC and (G) Phospho-MLC normalized to the β-actin. (H) Phospho-MLC was normalized to corresponding MLC protein band, respectively. Bars represent mean ± SEM, n = 5 homogenates/group, *P < 0.05 vs. CON group (by using student t-test).