Figure 4
From: Role of ROCK upregulation in endothelial and smooth muscle vascular functions in diabetic rat aorta
Effect of NO synthase inhibition with L-NAME on the relaxation response to Y27632 in the precontracted endo(+) aortic rings. (A) DM endo(+) aortic rings were exhibited an increased vasodilator sensitivity to Y27632 compared with control (CON) aortic rings. In the presence of L-NAME, ROCK inhibition was less effective to both DM and CON groups. Log [IC50] values on the relaxation to Y27632 are given in the inset. (B) Hyperglycemic (HG) endo(+) aortic rings were also exhibited an increased vasodilator sensitivity to Y27632 compared with CON aortic rings. Their Log [IC50] values on the relaxation to Y27632 are given in the inset. (C) Effect of ROCK inhibition on relaxation response to SNP after submaximal contraction with 1-μM Phe in endo(−) aortic rings were presented. In the presence of Y27632 an increased and equivalent vasodilatory sensitivity to SNP was observed for both DM and CON aortic samples. The Log [IC50] values for the relaxation to SNP are given in the inset. Data are expressed as mean ± SEM, n = 5-6/group. *P < 0.05 vs. CON group, by using student’s t test.