Figure 4

Triptolide attenuated HG-induced inflammation in H9c2 cells. (A, B) mRNA expressions of TNF-α and NF-κB p65, as determined by quantitative real-time RT-PCR. (C, D) protein expressions of TNF-α and NF-κB p65, as determined by western blot. (E) DNA-binding activity of free NF-κB p65 in nuclear extracts, as determined by TransAM™ NF-κB p65 Elisa. *P < 0.05 versus control; ^#P < 0.05 versus HG, n = 6 per group.