Expression levels of iNOS protein and Nos2 gene in hearts. (A) Representative immunoblots of iNOS and actin in hearts. (B) Quantified iNOS in heart normalized to actin levels. (C) mRNA expression levels of Nos2 expressed in cycle threshold values (Ct) normalized to the house-keeping gene Arbp. ZL: Zucker lean rats; ZDF: Zucker Diabetic Fatty rats. The number of experiments was 11 and 10 for the ZL and ZDF groups respectively.