Skip to main content
Figure 3 | Cardiovascular Diabetology

Figure 3

From: Heparanase induced by advanced glycation end products (AGEs) promotes macrophage migration involving RAGE and PI3K/AKT pathway

Figure 3

AGEs up-regulates HPA mRNA, protein expression and secretion in macrophages via RAGE. Cells were cultured with AGEs for 24 h with or without pre-treatment with antibody against RAGE for 1 h. A, The levels of HPA mRNA were assessed with real time quantitative RT-PCR. B, The secretion of HPA in supernatant was measured by enzyme-linked immunosorbent assay (ELISA). C, The expression of HPA protein in macrophages was determined by Western blotting. The results represent the mean of six culture wells (mean ± SEM). *p < 0.05 compared to control and #p <0.05 compared to AGEs. All of the experiments were performed independently in triplicate.

Back to article page