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Figure 2 | Cardiovascular Diabetology

Figure 2

From: High-density lipoprotein of patients with Type 2 Diabetes Mellitus upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in endothelial cells: relationship with HDL-associated sphingosine-1-phosphate

Figure 2

Diabetic HDL induces HUVEC COX-2 expression and PGI-2 release. Cells were incubated with N-HDL (HDL isolated from normal controls, 30 μg/mL, n=15), D-HDL (HDL isolated from diabetes, 30 μg/mL, n=15) for 6 hours. Cells were cultured with PBS as a negative control. The expression of COX-2 was assayed by Western blot analysis (A and B). PGI-2 release was determined by competitive ELISA (C). D-F showing that the effects of HDL on HUVECs were in a concentration-dependent pattern. Data are expressed as the means ± SEM of three independent experiments. Student’s t test. * p < 0.05. *** p < 0.001.

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Cardiovascular Diabetology

ISSN: 1475-2840