Figure 4
Eplerenone decreased lipid steatosis and ceramides formation in ZDF hearts. (A) Left, FAT/CD36 isoforms (~90 kDa and ~53 kDa; solid bars and broken bars in the quantification graph, respectively) expression in ZL, ZDF and ZDF+eplerenone hearts. Right, FABP3, ACADl and ACADm mRNA expression. (B) Detection of intra-cellular lipid deposition (steatosis) in the rats by ORO staining. A detailed 5x magnification of the lipid droplets is shown in ZDF. (C) Protein levels of GPAT1 (~90 kDa) and DGAT1 (~52 kDa) in rat myocardia. (D) By UHPLC-MS, DAG, phosphatidyl-choline (PC), TAG, and ketosphingosine, sphingosine and sphinganine levels. *p < 0.05 and **p < 0.01 vs. ZL. †p < 0.05 and ††p < 0.01 vs. ZDF rats.