Relative expression of AT
in WT and AT
-KO mice. Efficiency of the PCR for AT1a and AT1b was determined using serial dilutions of mouse adrenal gland cDNA (A and B). Parallel lines of the amplification reactions demonstrate similar efficiency of both PCRs. Expression of AT1a and AT1b was measured by real-time RT-PCR in cardiac myocytes (C) and kidneys (D) of wild type and AT1a-KO (control and diabetic (STZ, 10 wk)) mice. Values are expressed as the fold change compared to control ± SEM. *P < 0.05 vs. control.