Figure 4

ViLVaL combination therapy improves hepatic lipid accumulation. Hepatic steatosis was evaluated by Oil Red O staining of liver tissues of db/db mice. Scale bars indicate 100 μm at × 200 magnification (A). Liver extracts were subjected to western blotting for HSP72, PPARα and Sirt1 (B). Hepatic lipid oxidation was evaluated by measuring Acox1 and Mcad mRNA expression. Values represent mean ± SEM, n = 6 – 8. *p < 0.05, **p < 0.01 compared with the CT group (C).