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Table 3 QRT-PCR

From: Metabolic syndrome influences cardiac gene expression pattern at the transcript level in male ZDF rats

Description [Gene symbol]

Acc. No.:

DNA MICROARRAY

QRT-PCR

Confirmed

Fold change

p value

Ratio (SD)

Fold change

Regulation

ADAM metallopeptidase with thrombospondin type 1 motif, 1 [Adamts1]

NM_024400

−7.16

0.1295

0.58 (0.09)

−1.72

down

yes

heat shock 70kD protein 1A [Hspa1a]

NM_031971

−3.59

0.0045

0.34 (0.06)

−2.94

down

yes

similar to 60 kDa heat shock protein mitochondrial precursor [Hsp60]

XR_085659

−3.11

0.0565

0.64 (0.10)

−1.56

down

yes

interleukin 18 [Il18]

NM_019165

−2.60

0.0066

0.91 (0.15)

−1.10

no change

no

ATPase, (Na+)/K+ transporting, beta 4 polypeptide [Atp1b4]

NM_053381

−2.60

0.0031

0.37 (0.06)

−2.70

down

yes

argininosuccinate synthetase [Ass]

NM_013157

−2.51

0.0007

0.29 (0.05)

−3.42

down

yes

myosin IXA [Myo9a]

NM_134335

−2.40

0.0018

0.90 (0.15)

−1.11

no change

no

glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) [Gcat]

NM_001024277

−2.22

0.0063

0.33 (0.03)

−3.07

down

yes

activating transcription factor 3 [Atf3]

NM_012912

−2.05

0.0044

0.35 (0.06)

−2.85

down

yes

similar to chondroitin sulfate GalNAcT-2 [RGD1563660_predicted]

ENSRNOT00000019778

−1.87

0.0170

0.92 (0.15)

−1.09

no change

no

S100 calcium binding protein A3 [S100a3]

NM_053681

−1.87

0.0281

1.74 (0.28)

1.74

up

no

sushi, nidogen and EGF-like domains 1 [Sned1]

NM_001167842

−1.83

0.0407

0.42 (0.07)

−2.36

down

yes

G protein-coupled receptor 37 [Gpr37]

NM_057201

−1.82

0.0297

1.08 (0.18)

1.08

no change

no

angiopoietin-like 4 [Angptl4]

NM_199115

1.78

0.0073

2.90 (0.47)

2.90

up

yes

glutathione S-transferase Yc2 subunit [Yc2]

NM_001009920

1.82

0.0017

2.21 (0.36)

2.21

up

yes

ribonuclease, RNase A family, 1 (pancreatic) [Rnase1]

NM_001029904

1.86

0.0039

2.68 (0.44)

2.68

up

yes

similar to high density lipoprotein-binding protein [RGD1564237_predicted]

ENSRNOT00000009811

1.97

0.0020

2.73 (0.44)

2.73

up

yes

3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2[Hmgcs2]

NM_173094

2.07

0.0036

2.42 (0.39)

2.42

up

yes

similar to tetracycline transporter-like protein [RGD1311900_predicted]

ENSRNOT00000017386

2.24

0.1671

1.29 (0.21)

1.29

no change

no

ubiquitin carboxy-terminal hydrolase L1 [Uchl1]

NM_017237

2.37

0.0144

2.50 (0.41)

2.50

up

yes

ATPase, H+/K+ transporting, nongastric, alpha polypeptide [Atp12a]

NM_133517

2.61

0.0061

3.06 (0.50)

3.06

up

yes

phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) [Pla2g7]

NM_001009353

2.74

0.0411

4.25 (0.69)

4.25

up

yes

cytosolic acyl-CoA thioesterase 1 [Cte1]

NM_031315

3.69

0.0028

3.16 (0.51)

3.16

up

yes