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Figure 1 | Cardiovascular Diabetology

Figure 1

From: Metabolic syndrome influences cardiac gene expression pattern at the transcript level in male ZDF rats

Figure 1

Experimental protocol (A) Male Zucker Diabetic Fatty (ZDF) rats and their lean controls were followed up from 6 weeks of age until 25 weeks of age. Body weight (BW), serum glucose (Se Glu), insulin (Insulin), cholesterol (Chol) and triglyceride (TG) levels were determined at week 6, 16 and 25. Oral glucose tolerance test (OGTT) was performed at week 16 and 25. At week 25, hearts and pancreata were isolated. Pancreata were frozen and homogenized to measure pancreatic insulin levels. Hearts were perfused according to Langendorff for 10 minutes using Krebs-Henseleit buffer. Then ventricular tissue was frozen and used for DNA microarray analysis and cardiac free 3-nitrotyrosine level measurements. Serum glucose (B, n=6-8) and insulin levels (C, n=6-8), HOMA-IR (D), animal weight (E), serum cholesterol (F, n=6-8) and triglyceride (G, n=6-8) shown at week 6, 16 and 25 in both lean and ZDF rats. Solid line: Lean; dashed line: ZDF. Values are means±SEM, *p<0.05.

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