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Table 6 Evaluation of mRNA by real time PCR

From: Diabetes mellitus activates fetal gene program and intensifies cardiac remodeling and oxidative stress in aged spontaneously hypertensive rats

Variables

SHR (n = 10)

SHR-DM (n = 10)

P value

SERCA

1.00 ± 0.71

1.79 ± 1.22

0.125

α-MyHC

1.00 ± 0.60

0.72 ± 0.45

0.294

β-MyHC

0.87 (0.70 – 1.17)

1.11 (0.78 – 1.28)

0.470

β-MyHC/α-MyHC

1.20 ± 0.60

2.26 ± 0.94

0.018

ANP

1.00 ± 0.38

1.88 ± 0.69

0.009

  1. Data are expressed as mean ± standard deviation or median and 25th and 75th percentile (arbitrary units). SHR: spontaneously hypertensive rats without diabetes mellitus; SHR-DM: SHR with diabetes mellitus. SERCA: sarcoplasmic reticulum calcium ATPase; MyHC: myosin heavy chain; ANP: atrial natriuretic peptide; Cyclophilin: housekeeping gene used in standardizating target gene transcript product quantity. P value: result of statistical test (Student’s t or Mann-Whitney tests).