Arg972 Insulin receptor substrate-1 is associated with decreased serum angiotensin-converting enzyme 2 levels in acute myocardial infarction patients: in vivo and in vitro evidence

Table 6 Relative mrna levels of angiotensin-converting enzyme 2 (ace2) in human cardiomyocytes under normoxia and hypoxia with or without insulin treatment

Note: Human cardiomyocytes were stably transfected with wild type (WT) IRS-1 or Arg⁹⁷² IRS-1, or stably transduced with IRS-1-shRNA. Then the cells were cultured under normoxia or hypoxia for 24 hours in the presence or absence of LY294002 (LY, 50 μM) or insulin (1 mg/L). Human cardiomyocytes stably transfected with empty pcDNA3.1 plasmids was used as a control (VC). *p<0.05 compared with any group under normoxia; ^ap<0.05 compared with VC or SC under hypoxia+insulin (1 mg/L); ^bp<0.05 compared with WT-IRS-1 under hypoxia+insulin (1 mg/L); ^cp<0.05 compared with Arg972-IRS-1 under normoxia+insulin (1 mg/L); ^dp<0.05 compared with Arg972-IRS-1 under hypoxia+insulin (1 mg/L).

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