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Figure 3 | Cardiovascular Diabetology

Figure 3

From: Arg972 Insulin receptor substrate-1 is associated with decreased serum angiotensin-converting enzyme 2 levels in acute myocardial infarction patients: in vivo and in vitro evidence

Figure 3

ADAM17 Shedding Activity in Human Cardiomyocytes under Normoxia and Hypoxia with or without Insulin. Human cardiomyocytes were stably transfected with wild type (WT) IRS-1 or Arg972 IRS-1, or stably transduced with IRS-1-shRNA. Then the cells were cultured under normoxia or hypoxia for 24 hours in the presence or absence of LY294002 (LY, 50 μM) or insulin (1 mg/L). Human cardiomyocytes stably transfected with empty pcDNA3.1 plasmids (VC) or stably transduced with scramble control shRNA (SC) were used as controls. The ADAM17 shedding activity was determined by ELISA and expressed as fold changes to that of the VC cells under normoxia+insulin (1 mg/L) (designated as 1). *p<0.05 compared with any group in normoxia.

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Cardiovascular Diabetology

ISSN: 1475-2840