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Figure 1 | Cardiovascular Diabetology

Figure 1

From: Arg972 Insulin receptor substrate-1 is associated with decreased serum angiotensin-converting enzyme 2 levels in acute myocardial infarction patients: in vivo and in vitro evidence

Figure 1

Western Blot Analysis of IRS-1 Expression in Human Cardiomyocytes. A , human cardiomyocytes were stably transfected with wild type (WT) IRS-1 or Arg972 IRS-1, or stably transduced with IRS-1-shRNA. Human cardiomyocytes stably transfected with empty pcDNA3.1 plasmids (VC) or stably transduced with scramble control shRNA (SC) were used as controls. Cell lysates were subject to western blot analysis using an anti-IRS-1 antibody. β-Actin blotting was used as a loading control. B , protein blots were measured by densitometry. The density of the blots was normalized against that of β-actin to obtain a relative blot density. *p<0.05 compared with VC.

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