Interferon regulatory factor-1 together with reactive oxygen species promotes the acceleration of cell cycle progression by up-regulating the cyclin E and CDK2 genes during high glucose-induced proliferation of vascular smooth muscle cells

Table 1 Analysis of VSMCs cycle distribution by flow cytometry

		Untransfected VSMCs (%)	VSMCs transfected with pGC-FU (%)	VSMCs transfected with pGCsi-FU-Irf-1 (%)	VSMCs transfected with pGC-FU-Irf-1 (%)
High glucose	G0/G1	64.8 ± 2.7	65.0 ± 2.9	82.5 ± 3.2*	51.0 ± 2.4*
	S	21.2 ± 1.2	20.9 ± 1.4	10.5 ± 1.1*	29.8 ± 1.6*
	G2/M	13.9 ± 1.3	14.1 ± 1.7	6.9 ± 0.9*	19.3 ± 1.7*
High glucose/NAC	G0/G1	69.5 ± 2.7	69.9 ± 3.0	69.4 ± 1.3^#	71.1 ± 3.4^#
	S	18.7 ± 1.6	19.1 ± 1.2	18.3 ± 1.5^#	17.9 ± 2.1^#
	G2/M	11.7 ± 1.5	11.1 ± 1.4	12.3 ± 1.3^#	10.9 ± 2.1^#
Normal glucose	G0/G1	80.8 ± 3.3	80.3 ± 2.9	81.5 ± 3.7	88.4 ± 3.5*
	S	11.8 ± 1.2	12.2 ± 1.3	11.3 ± 1.0	7.3 ± 1.1*
	G2/M	7.3 ± 1.1	7.5 ± 1.0	7.3 ± 1.2	4.2 ± 0.7*
Normal glucose/H₂O₂	G0/G1	72.0 ± 2.9^	73.4 ± 3.1^	82.5 ± 3.2*	55.5 ± 2.3*^
	S	17.1 ± 2.2^	16.8 ± 1.7^	11.3 ± 1.2*	27.9 ± 2.3*^
	G2/M	10.8 ± 1.3^	9.9 ± 1.7^	6.1 ± 1.0*	16.7 ± 1.9*^
High glucose/U0126	G0/G1	71.3 ± 2.8^#	72.5 ± 3.1^#	70.6 ± 1.9^#	69.8 ± 3.6^#
	S	17.9 ± 1.6^#	17.4 ± 2.0^#	16.5 ± 1.6^#	18.1 ± 1.9^#
	G2/M	10.9 ± 1.7^#	10.2 ± 1.5^#	12.8 ± 2.8^#	12.0 ± 2.6^#
Normal glucose/H₂O₂/U0126	G0/G1	70.3 ± 2.1^	68.9 ± 3.6^	69.8 ± 2.3^	70.9 ± 3.2^
Normal glucose/H₂O₂/U0126	S	17.5 ± 1.8^	18.5 ± 1.6^	18.4 ± 1.7^	17.8 ± 2.1^
	G2/M	12.1 ± 1.7^	12.5 ± 1.5^	11.9 ± 1.4^	11.4 ± 1.9^

Values are mean ± SD from triplicate determinations repeated in 4 separate experiments.
*P < 0.05 vs. corresponding values in untransfected VSMCs; ^P < 0.05 vs. corresponding values in VSMCs under normal glucose; ^#P < 0.05 vs. corresponding values in VSMCs under high glucose.

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