Figure 8

Mitochondrial membrane polarization in HUVEC and HMVEC following high glucose treatment for 24 hours. Using JC-10 mitochondrial membrane polarization was measured in both HUVEC and HMVEC after 24 hours of high glucose treatment. Mitochondrial membrane polarization of high glucose treated HUVEC was evaluated through measurement of JC-10 aggregates (Red) and monomers (Green) using fluorescence microscopy and microplate reader. a) Overlapped fluorescence images of red and green fluorescence intensities along with Hoescht-33342 based nuclear stain were used to show hyperpolarized mitochondrial membrane in high glucose treated HUVEC. b) Similarly, ratio of red and green fluorescence intensities were plotted to illustrate mitochondrial membrane hyperpolarization in high glucose treated HUVEC. **p ≤ 0.05 significantly higher than 5.6 mM D-glucose exposure treatment in HUVEC. c) Overlapped fluorescence images of red and green fluorescence intensities along with Hoescht-33342 based nuclear stain were used to show depolarized mitochondrial membrane in high glucose treated HMVEC. d) Ratio of red and green fluorescence intensities were plotted to show high glucose exposure-induced mitochondrial membrane depolarization in HMVEC. **p ≤ 0.05 significantly higher than 25 mM D-glucose exposure concentration in HMVEC.