‾ production in HUVEC and HMVEC cells following high glucose exposure for 24 hours. Using DHE levels of O2‾ were measured in both HUVEC and HMVEC following 24 hours of high glucose treatment. Fluorescence imaging was used to capture DHE fluorescence in (a-b) HUVEC and (c-d) HMVEC. Fluorescence microplate reader based measurements were also performed to measure specific fluorescence intensity of 2-OH-E+ in (e) HUVEC and HMVEC treated with high glucose. For both types of DHE measurements, cells grown in 5.6 mM D-glucose were used as control. **p ≤ 0.01 significantly higher than control for respective cells type.