Figure 4
Expression levels of peroxide clearance enzymes in HUVEC, and HMVEC cells after 24 hours of high glucose exposure. Gene expression of GPX1, TXNRD1, TXNRD2, and PRDX1 were measured in (a) HUVEC and (b) HMVEC following 24 hours of exposure to HG-M199. Fold changes were calculated by 2-∆∆CT method using 18s and RPLP0 as housekeeping genes and cells grown in M199 media with 5.6 mM D-glucose as control. **p ≤ 0.05 significantly higher than control for HMVEC.
