Figure 3
Expression levels of ROS clearance enzymes in HUVEC, and HMVEC cells after 24 hours of high glucose exposure. Gene expression of SOD1, SOD2, and CAT were measured in (a) HUVEC and (b) HMVEC following 24 hours of treatment with HG-M199. Fold changes were calculated by 2-∆∆CT method using 18s and RPLP0 as housekeeping genes and cells grown in M199 media with 5.6 mM D-glucose as control. **p ≤ 0.05 significantly different than control for respective cells type.