Figure 2

Effect of different inhibitors on the expression of profilin-1 induced by AGEs in endothelial cells. A: the protein expression of profilin-1 by western blotting; B: the mRNA expression of profilin-1 by real-time PCR. 1: control; 2: AGEs (200 μg/ml): endothelial cells were incubated with 200 μg/ml AGEs for 24 h; 3: A+DPI (10 μM): endothelial cells were incubated with 10 μmol/L DPI for 1 h prior to AGEs (200 μg/ml, 24 h); 4: A+BAY-117802 (5 μM): endothelial cells were incubated with 5 μmol/L BAY-117802 for 1 h prior to AGEs (200 μg/ml, 24 h); 5: A+GF 109203X (10 μM): endothelial cells were incubated with 10 μmol/L GF 109203X for 1 h prior to AGEs (200 μg/ml, 24 h); 6: A+si profilin-1 (4 μg): endothelial cells were incubated with 200 μg/ml AGEs and 4 μg pLNCX2-si profilin-1 for 4 h and then with 200 μg/ml AGEs for 20 h; n=3, ^**P < 0.01 vs control, ^#P < 0.05 vs AGEs (200 μg/ml), ^##P < 0.01 vs AGEs (200 μg/ml).