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Figure 3 | Cardiovascular Diabetology

Figure 3

From: Methylglyoxal modulates endothelial nitric oxide synthase-associated functions in EA.hy926 endothelial cells

Figure 3

Effect of methylglyoxal on cellular biopterin levels in EA.hy926 endothelial cells. A and B. Total cellular biopterin (pmol/mg protein; black bars; A) and BH4 (pmol/mg protein; white bars; A) and the respective BH4/total biopterin ratio (B) determined in EA.hy296 endothelial cells treated with different concentrations of MG (0, 50, 100 and 200 μM) for 8 h. Data are arithmetic means ± SEM (n = 5). * indicates significant difference (p < 0.05) from the absence of MG (ANOVA). C and D. Total cellular biopterin (pmol/mg protein; black bars; C) and BH4 (pmol/mg protein; white bars; C) and the respective BH4/total biopterin ratio (D) determined in EA.hy296 endothelial cells treated with MG (100 μM) for 8 h in the absence (Control) or in the presence of L-NAME (50 μM), BH4 (100 μM), NH4 (100 μM) or sepiapterin (20 μM) in the last 4 h of MG treatment or with 24-h pre-treatment with 300 μM Tempol and 50 μM L-NAME (ONOO- suppression). Data are arithmetic means ± SEM (n = 5). * indicates significant difference (p < 0.05) from Control (ANOVA).

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