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Figure 2 | Cardiovascular Diabetology

Figure 2

From: Methylglyoxal modulates endothelial nitric oxide synthase-associated functions in EA.hy926 endothelial cells

Figure 2

Methylglyoxal- induced eNOS monomerization in EA. hy926 endothelial cells. A and B. Original Western blots (A) and the respective densitometric analysis (B) of the relative abundance of eNOS dimers over eNOS monomers (relative to β-actin) in EA.hy296 endothelial cells treated with different concentrations of MG (0 μM, circles; 50 μM, triangles; 100 μM, squares; and 200 μM, diamonds) for 0.5, 2, 4, and 8 h respectively. Data are arithmetic means ± SEM (A, representative of five experiments; B, n = 5). * indicates significant difference (p < 0.05) from the absence of MG (ANOVA). # indicates significant difference (p < 0.05) from time 0.5 h (ANOVA). C and D. Original Western blots (C) and the respective densitometric analysis (D) of the relative abundance of eNOS dimers over eNOS monomers (relative to β-actin) in EA.hy296 endothelial cells incubated in the absence (-) or in the presence of MG (100 μM) for 8 h alone (+) or with 100 μM BH4, 100 μM NH4, 20 μM sepiapterin or 50 μM L-NAME in the last 4 h of MG treatment or with 24-h pre-treatment with 300 μM Tempol and 50 μM L-NAME (ONOO- suppression). Data are arithmetic means ± SEM (C, representative of four experiments; D, n = 4). * indicates significant difference (p < 0.05) from the absence of MG (ANOVA). # indicates significant difference (p < 0.05) from MG treatment alone (ANOVA).

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