Figure 4

Effects of propofol on 15 mM glucose-induced nuclear factor kappa B (NF-κB) activation. (A) Human umbilical vein endothelial cells were cultured in either 5 mM glucose or 15 mM glucose for 4 h with corresponding treatment. Shifted band was increased by 15 mM glucose, but reduced by propofol (n = 5). (B) In the supershift assay, 15 mM glucose-induced band was shifted by the pre-incubation of nuclear extracts with anti-NF-κB antibody, but was not affected by normal rabbit IgG (n = 5). (C) In the competition assay, 100-fold excess of nonradiolabeled probes were incubated with nuclear extracts 10 min before the addition of radiolabeled probes. 15 mM glucose-induced shifted band was blocked by nonradiolabeled competitors, but mutant competitors had no such effect (n = 5).