Figure 6
Insulin-stimulated peripheral tissue glucose utilization. (a) Metabolic index of glucose uptake (R g ) in the soleus. (b) Metabolic index of glucose uptake (R g ) in the superficial vastus lateralis. (c) Metabolic index of glucose uptake (R g ) in the gastrocnemius. (d) Metabolic index of glucose uptake (R g ) in white adipose tissue. Tissue R g values are relative to brain R g . n = 9 mice per group. (e) Gastrocnemius glucose transporter 4 (GLUT4), phospho-Akt (p-Akt), Akt and p-Akt-to-total Akt ratio (p-Akt/Akt) as determined by immunoblotting. n = 6-8 mice per group. (f) Representative immunoblotting performed to measure gastrocnemius GLUT4, p-Akt and Akt. (g) White adipose tissue glucose transporter 4 (GLUT4), phospho-Akt (p-Akt), Akt and p-Akt-to-total Akt ratio (p-Akt/Akt) as determined by immunoblotting. n = 6 mice per group. (h) Representative immunoblotting performed to measure white adipose tissue GLUT4, p-Akt and Akt. Protein levels are normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) content and are expressed relative to the SHAM group. Data are mean ± S.E.M. *p < 0.05 vs. SHAM. †p < 0.05 vs. MI + PBS.