Figure 5

Gel electrophoresis of LV extracts from treatment groups of rats. Gels were stained with Pro-Q Diamond (for phosphoproteins) and SYPRO^® Ruby (for total protein). Upper panels show representative gels (n = 3/group). Lower panels show graphical representation of the results of densitometry: (i) Diabetic animals (D, n = 6, solid bars) showed increased proportions of phosphorylated TnI (expected molecular weight, 28 kDa) compared with controls (C, n = 6, open bars) whereas TETA-treated diabetic animals (DT, n = 6, patterned bars) showed levels comparable to control values. (ii) Neither diabetes nor TETA treatment modified the phosphorylation of TnT (expected molecular weight, 40–42.5 kDa). Data in lower panels are means ± SEM analysed by one-way ANOVA (P = 0.005) with post-hoc Tukey’s Multiple Comparisons tests: *, C vs D, P < 0.01; †, D vs DT, P < 0.05. Abbreviations: TnI, troponin I; TnT, troponin T.