TECs isolated from kidney explants express renal tubular markers and produce Klotho. (A) Cultured TECs form a monolayer containing cells with heterogeneous morphology. Immunofluorescence revealed extensive immunoreactivity with antibodies against epithelial markers Pan-cytokeratin (AE1/3) (B), Cytokeratin 7 (CK7) (C) and Epithelial Membrane Antigen (EMA) (D). Moreover, TECs produce Klotho as shown with immunofluorescence (E) and immunohistochemistry (F). Nuclear staining is shown in blue while positive staining with antibodies is shown in red (AlexaFluor®555 for immunofluorescence and AEC for immunohistochemistry). Arrows indicate Klotho positive epithelial cells. BF: Brightfield. Magnification: 200x; magnification, insets: 630x.