The impact of type I diabetes on the neurogenic response of cardiac resident nestin(+)cells during scar formation/healing of the myocardial infarcted rat heart. (Panels A, B &C) Neurofilament-M(+) (green fluorescence) and nestin(+) (red fluorescence) fibres were detected innervating the peri-infarct/infarct region of a post-MI rat heart and co-expression was evident by the emergence of a yellow fluorescence (indicated by arrow). Neurofilament-M(+) fibres lacking nestin co-expression were also identified (indicated by arrowhead). (Panels D, E &F) The reparative fibrotic response of diabetic post-MI rats was associated with a paucity of innervating neurofilament-M/nestin(+) fibres (indicated by arrow) in the peri-infarct/infarct region. Nestin(+) cardiac myocyte-like cells were detected bordering the scar region and residual nestin(+) cells and fibres were identified in the peri-infarct/infarct region.