Nestin and GAP43 protein expression in non-diabetic and diabetic infarcted rat hearts. (Panel A). Nestin expression was increased in the non-infarcted left ventricle of myocardial infarcted (MI) rats. In streptozotocin (STZ) induced diabetic rats, nestin protein expression was diminished as compared to sham rats. Ventricular nestin protein levels remained reduced following the superimposition of type I diabetes on 3-day post-MI rats (MI + STZ) as compared to post-MI rats. Ventricular GAP43 protein levels were comparable in sham, diabetic, post-MI and diabetic post-MI rats. (Panel B) GAP43 expression was similar in the scar of myocardial infarcted (MI) and diabetic post-MI rats (MI + STZ). (Panel C) Semi-quantitative assessment of nestin and GAP43 expression in the left ventricle of sham (n = 4-5) and diabetic rats (STZ; n = 4) and the non-infarcted left ventricle of post-MI (MI; n = 4) and diabetic post-MI rats (MI + STZ; n = 6). Data was normalized to GAPDH protein expression, (*) denotes P < 0.05 versus sham and (**) P < 0.05 versus MI.