Skip to main content
Figure 9 | Cardiovascular Diabetology

Figure 9

From: Exercise and diabetes have opposite effects on the assembly and O-GlcNAc modification of the mSin3A/HDAC1/2 complex in the heart

Figure 9

Effects of diabetes and 4 weeks of exercise on HDAC1 activation and the mSin3A/HDAC1 interaction. (A) Immunoprecipitation (IP) of HDAC1 and immunoblot for its association with mSin3A in ventricular tissue from C57BL/6J (C57) and db/db (db) mice that were treadmill exercised (Ex) or sedentary (Sed) for 4 weeks. (B) Immunoprecipitation (IP) of HDAC1 and immunoblot for phospho-serine 421/423, which activates HDAC1. (C-D) Blot quantification of (A) and (B). (E) Colorimetric assay quantification of class 1 HDAC activity in ventricular tissue. N = 6 per group for HDAC1 immunoprecipitation; N = 3 per group for HDAC1/2 activity assay. * Significant effect of genotype within an exercise group, P < 0.05. # Significant main effect of genotype, P < 0.05.

Back to article page