Figure 2

1 week of exercise increases total protein O-GlcNAcylation in db/db mouse hearts. (A) Western blot and (B-G) quantification of total protein O-GlcNAcylation in ventricular tissue from C57BL/6J (C57) and db/db (db) mice that were exercised (Ex) or sedentary (Sed) for 1 week. O-GlcNAc levels are normalized to the loading control calsequestrin (Csq). N = 6 per group. * Significant effect of genotype within an exercise group, P < 0.05. # Significant effect of exercise within a genotype group, P < 0.05. ‡ Significant interaction of exercise x genotype, P < 0.05.