Table 1 Primers used in semi-quantitative reverse transcription-PCR
| Gene symbol | primer (5′→3′) | Product size (bp) |
|---|---|---|
| AdipoR1 | ||
| Sense | 5′-TCTTCCTCATGGCTGTGATG-3′ | 190bp |
| Antisense | 5′-TCTAGGCCGTAACGGAATTC-3′ | |
| AdipoR2 | ||
| Sense | 5′-ACCCACAACCTTGCTTCATC-3′ | 233bp |
| Antisense | 5′-GCTAGCCATGAGCATTAGCC-3′ | |
| GLUT4 | ||
| Sense | 5′-GATGCCGTCGGGTTTCCAGCA-3′ | 233bp |
| Antisense | 5′-TGAGGGTGCCTTGTGGGATGG-3′ | |
| MCP-1 | ||
| Sense | 5′-CTGTCTCAGCCAGATGCAGTT-3′ | 147bp |
| Antisense | 5′-GAGCTTGGTGACAAATACTACA-3′ | |
| GAPDH | ||
| Forward | 5′-TGAACGGGAAGCTCACTGG-3′ | 307bp |
| Reverse | 5′-TCCACCACCCTGTTGCTGTA-3′ |