Real-time fluorescence quantitative PCR analysis of myocardial mRNA expression of NOX4 in control and diabetic rats treated with or without telmisartan. Relative gene expression levels were normalized relative toβ-actin. Control (C), diabetic (D), diabetic treated (DT). Data are expressed as mean ± S.E.M (n = 10 per group). * P <0.05 different from control, # P <0.05 different from diabetic.